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BINDS
Expression, Functional Analysis, and In Silico Analysis Collaborative Unit

Spatial expression analysis equipment

10x Genomics

Xenium

Spatial analysis of subcellular resolution

Capture area

up to 12 x 24 mm

Corresponding Samples

Fresh frozen, FFPE

Number of detectable genes

Xenium v1: Maximum 480(default) + 100(customizable)

Xenium Prime 5K: 5000(default) +100(customizable)

10x Genomics

Visium CytAssist

全トランスクリプトームによる空間解析

Capture area

6.5 x 6.5 mm or 11 x 11 mm

Corresponding Samples

Fresh frozen, Fixed frozen, FFPE

Compatible species

Visium HD: Human/Mouse
Visium v2: Widely compatible

Spatial analysis using the entire transcriptome

By placing tissue onto a capture area lined with spatially identified probes, a sequence-based, full transcriptome analysis linked to spatial information is performed.
To understand complex tissues, neighboring regions, and intercellular interactions,
It can be integrated with information on other spatial omics, tissues, and morphologies.
Observation using H&E staining or immunofluorescence staining is possible with the same specimen.

10x Genomics

Chromium

High-throughput single-cell analysis

Capture amount

Up to 10,000 cells per channel

Corresponding assay

Single-cell gene expression, single-cell immune profile, single-cell gene expression Flex
Single-cell multi-ohm ATAC + gene expression Single-cell ATAC
Single-cell gene expression HT
Single-cell immune profile HT

AKOYA Bioscience

Phnobucycler

Spatial single-cell proteome analysis in the tissue microenvironment

Corresponding Samples

Fresh frozen, FFPE

Laboratory made

Micro tissue sampling device

Spatial analysis using the entire transcriptome

sequencer

Oxford Nanoins Technologies

GridION

Benchtop DNA/RNA sequencer

PacBio

Revio

Long-read sequencer

Long-read sequencer

HiFi sequencing using the Revio system,
Tens of thousands of base pairs of HiFi reads were obtained.
It offers exceptional sequencing accuracy, enabling direct detection of DNA methylation and acquisition of reads with uniform coverage regardless of the context within the sequence.
Epigenetic analysis, repeat sequence analysis, and isoform analysis are possible.
In addition, target sequencing is possible.

Illumina

Nextseq 2000

Short-read sequencer

Element

AVITI

Short-read sequencer

Other equipment

Agilent Technologies

Femto Pulse

Fully automated pulsed-field parallel capillary electrophoresis system

Bigfoot Spectral Cell Sorter

Thermo Fisher Scientific

High-throughput cell sorter

4200 TapeStation

Agilent Technologies

Fully automated high-throughput electrophoresis system

All-in-one fluorescence microscope
BZ-X800a

KEYENCE

All-in-one fluorescence microscope

Mica

Leica Microsystems

All-in-one imaging system

GentleMACS™ Dissociators

Miltenyi Biotec

Automated tissue dispersion and crushing device

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